外泌體研究 Exosome Research
外泌體是由細胞釋放的小囊泡，直徑約 30–150 nm，載有蛋白、核酸、脂質，與細胞間通訊、疾病標記、液體活檢等研究密切相關。Novus Biologicals提供從純化、標定到定量的全套產品，包括標記抗體、抗體組合包 (Antibody Pack)、ELISA 檢測套組，以及純化 / 富集試劑，協助完成外泌體樣本製備與分析流程。
產品分類
外泌體分離工具
Exosome Isolation Tools
外泌體標準品/螢光標準品
Exosome Standard
外泌體標記抗體
Exosome Detection Antibody pack
外泌體定量工具
Human Exosome ELISA kit
外泌體標準品/螢光標準品
Characterization Tools
外泌體相關文獻與應用資源
Literature and Applications
★ 熱門產品推薦 ★
| Exosome isolation | ||||
| Cat No. | Product Name | Sample Type | Sample Volume | Principle |
| NBP3-11765 | EV Precipitation solution (Blood) | Plasma & Serum | Plasma: 100–250 µL, Serum: 250–500 µL | Precipitation |
| NBP3-11766 | EV Precipitation solution (Urine) | Urine | 5–20 mL | |
| NBP3-11767 | EV Precipitation solution (Cell Culture) | Cell culture supernatant | 1–5 mL | |
| NBP3-11762 | TFF-EV ConcentratorRemove < 100 kDa | Diluted matrices (urine and cell culture supernatant) |
Cell culture supernatant: 5–1000 m, LUrine: ≤1000 mLPlasma/serum: 5–20 mL |
Ultrafiltration |
| NBP3-26837 | TFF-Small EV IsolationRemove < 800 kDa | Urine, cell culture supernatant, plasma, serum | 10 mL – 1000 mL | |
| NBP3-26838 | TFF-Large EV IsolationRemove < 800 kDa | Urine, cell culture supernatant, plasma, serum, plant extracts |
500 mL – 10000 mL | |
| NBP3-11763 | EV SEC Columns | Biofluid and cell culture supernatant | 0.5–2 mL | Size Exclusion Chromatography (SEC) |
| NBP3-11764 | EV MaxiSEC Columns | Urine, cell culture supernatant, or other diluted matrices |
5–20 mL | |
| NBP3-11768 | CD9 Immunobeads for Exosome Isolation | Human biofluid | 0.5–1 mL |
Immuno affinity |
| NBP3-11769 | CD63 Immunobeads for Exosome Isolation | Human cell culture supernatant | 0.5–1 mL | |
| Exosome standard | |||
| Source | Sample/Cell line | Lyophilized | Fluorescent |
| Healthy Biofluid | Human serum | NBP2-49827 | NBP3-11693 |
| Human Urine | NBP2-49840 | NBP3-11694 | |
| Human plasma | NBP3-11686 | NBP3-11692 | |
| Healthy human cell line | HEK293 | NBP3-11684 | - |
| (Embryonic kidney cells) | |||
| Human Glioma (GBM) | U87 MG | NBP2-49844 | NBP3-11698 |
| Human Neuroblastoma | SK-N-SH | NBP2-49852 | NBP3-11697 |
| Human colon cancer | COLO1 | NBP2-49845 | NBP3-11690 |
| HCT116 | NBP2-49854 | NBP3-11691 | |
| Human prostate cancer | PC3 | NBP2-49856 | NBP3-11696 |
| LnCAP | NBP3-11687 | - | |
| Human lung cancer | A549 | NBP3-11645 | NBP3-11699 |
| NCI-H1975 | NBP3-11688 | - | |
| Human leukemia (CML) | K-562 | NBP2-49864 | NBP3-11695 |
| Human colorectal cancer | HT29 | NBP3-11685 | - |
| Mouse melanoma | B16F10 | NBP2-49866 | NBP3-11689 |
Application of Lyophilized Exosome Standards
|
Marker detection by Western Blotting |
Phenotyping by ELISA assay |
|
NTA in fluorescence and scattered mode |
Phenotyping assays by FACS |
|
Profiling of Exosome associated nucleic acids |
| Exosome Detection (Western Blot) Antibody Pack Cat# NBP3-11740 | |
| Component | Meaning |
| CD9 Antibody (1021007)[Unconjugated] | Positive marker: Tetraspanins (CD9, CD63, and CD81) |
| CD63 Antibody (H5C6) – BSA Free | Positive marker: Tetraspanins (CD9, CD63, and CD81) |
| CD81 Antibody (1D6) – BSA Free | Positive marker: Tetraspanins (CD9, CD63, and CD81) |
| TSG101 Antibody – BSA Free | Positive marker: Intracellular protein associated with multivesicular body (MVB) formation |
| HSP70/HSPA1A Antibody (242707)[Unconjugated] | Positive marker: Chaperone protein |
| Calnexin Antibody (3H4A7) | Negative marker: Contamination of exosome prep with secretory vesicles (Calnexin) |
| Albumin Antibody (188835)[Unconjugated] – Serum | Negative marker: Contamination of exosome prep with circulating free protein (albumin) |
| Human Exosome ELISA kit (Colorimetric) | |||
| Cat No. | Product Name | Sample Type | Sample Volume |
| NBP3-11770 | Human Exosome (Plasma) ELISA Kit (Colorimetric) | Human plasma | 10uL |
| NBP3-11771 | Human Exosome (Serum) ELISA Kit (Colorimetric) | Human serum | |
| NBP3-11772 | Human Exosome (Cell Media) ELISA Kit (Colorimetric) | Cell culture supernatant (pre concentrated) | |
| NBP3-11773 | Human Tumor-derived Exosome ELISA Kit (Colorimetric) | Human plasma | |
| NBP3-14800 | Human Exosome (Urine) ELISA Kit (Colorimetric) | Human Urine | |
WARNING! Do not use with plasma heparin samples!
1: Example of standard curves: CD9 Titration of COLO1
(1) and plasma (2) Exosome Standards. This standard curve is fordemonstration only.
For quantification purposes a standard curve must be obtained with every assay.